ypgal medium  (Difco)

Journal: Cells

Article Title: Functional Analysis of the Ribosomal uL6 Protein of Saccharomyces cerevisiae

doi: 10.3390/cells8070718

Figure Lengend Snippet: Growth of uL6 mutant yeast strains on various carbon sources. ( A , B ) Cells of the Δul6A, Δul6B , and GAL::uL6A mutants and the wild type were spotted onto agar plates with YPD (Glucose) or YPGal (Galactose) medium as indicated and incubated at 30 °C for 3 days. ( C ) Growth curves of GAL::uL6A (red squares) and wild type (blue diamonds) strains at 30 °C after shifting exponential cultures from liquid YPGal to liquid YPD medium. The optical density (OD 600 ) of the cultures was measured at different time points for up to 12 h. Error bars represent standard deviations obtained from three independent experiments. ( D – F ) Effects of depletion of both uL6A and uL6B on the budding ability and viability. GAL::uL6A mutant cells were grown on YPD ( D ) or YPGal ( E ) plates, and cell growth was monitored under the microscope after 0 h, 24 h, and 72 h. ( F) Propidium iodide staining of GAL::uL6A budding yeast cells.

Article Snippet: For verification of the cells budding, 20 µL of the cell suspensions were spotted on the plate with solid YPD or YPGal medium, and the pictures of the cells were taken using the Nikon Eclipse E200 microscope equipped with the Olympus DP26 digital camera at the beginning of the experiment and after 0 h, 24 h, and 48 h. For determining death cells, staining with PI was used.

Techniques: Mutagenesis, Incubation, Microscopy, Staining

Journal: Cells

Article Title: Functional Analysis of the Ribosomal uL6 Protein of Saccharomyces cerevisiae

doi: 10.3390/cells8070718

Figure Lengend Snippet: Polysome profile analysis of uL6 mutants. Polysome profile of ( A ) single uL6A or uL6B deletion mutants and wild type strain grown on YPD; ( B ) double uL6A and uL6B mutant grown on YPD for 12 h and 24 h after the shift from the YPGal medium. The sedimentation vector of the ribosomal fractions is indicated by a horizontal arrow, and optical density analysis at 254 nm is shown on the Y -axis; the positions of individual ribosomal subunits and half-mers are indicated.

Article Snippet: For verification of the cells budding, 20 µL of the cell suspensions were spotted on the plate with solid YPD or YPGal medium, and the pictures of the cells were taken using the Nikon Eclipse E200 microscope equipped with the Olympus DP26 digital camera at the beginning of the experiment and after 0 h, 24 h, and 48 h. For determining death cells, staining with PI was used.

Techniques: Mutagenesis, Sedimentation, Plasmid Preparation